2019-05-10
New feature (and breaking change):
compute_all_nearest_distance and find_nearest_distance now create columns containing the Cell ID of the nearest cell, as well as the columns with the actual distance. This column can be used to find the locations of nearest cells and to find mutual nearest neighbors. The “Computing inter-cell distances” tutorial shows some uses of this data.Bug fixes:
read_cell_seg_data recognizes and correctly reads inForm data which uses comma as the decimal separator (#8).count_touching_cells ignores phenotype pairs of self to self with a warning because the touching algorithm does not handle this case (#7).2019-04-22
Failed with error: ‘there is no package called ‘rtree’’ message that requireNamespace('rtree') generates (#5).2019-04-12
New features:
count_within_many and count_within_batch using akoyabio/rtree package.count_within_detail to give per-cell counts.Bug fixes:
get_field_info to work with the standard (CRAN) tiff package.count_within and related functions return NA for within_mean when there are no from cells (rather than a mean of 0).spatial_distribution_report vignette and example to use phenoptrExamples, which contains the required component data file (#3).count_touching_cells when there is exactly one touching cell pair (#4)2019-03-03
count_within_many in support of phenoptrReports.count_within does not count self when ‘from’ and ‘to’ phenotypes are the same.field_column (moved from phenoptrReports) and validate_phenotypes.compute_all_nearest_distance to recognize “Annotation ID” as a field name.2019-02-08
find_nearest_distance, compute_all_nearest_distance, density_at_distance, density_bands, count_within and spatial_distribution_report to work with consolidated data from phenoptrReports (#1).Bug fixes
sq microns and sq mm to square microns and square mm to match inForm output.2018-12-27
spatial_distribution_report works correctly with cell seg data in microns. To do so, it requires a component data file for the target field. Image dimensions are taken from that file.count_touching_cells works correctly when cell seg data is in microns.read_cell_seg_data #9
count_within won’t complain if the data doesn’t have a Sample Name column.2018-09-28
2018-09-28
parse_phenotypes function simplifies creation of selectors for select_rows.select_rows recognizes the column format of phenotype-per-marker data.select_rows treats a NA selector as “select all”. This is helpful in lists of selectors.read_cell_seg_data attempts to skip microns conversion if it was already done.2018-07-11
count_touching_cells to work with two-pixel-wide membrane map. #82018-04-24
count_within_batch to work with cell seg files which don’t have Slide ID fields.density_at_distance and density_bands to estimate cell density at a distance from a boundary.pixels_per_micron="auto" option to read_cell_seg_data().informr to phenoptr.subset_distance_matrix in a backwards-incompatible way. This was done to put the csd parameter first, matching other functions with a csd parameter.spatial_distribution_report creates an HTML report showing the location and nearest-neighbor relations of cells in a single field.count_touching_cells uses morphological analysis of nuclear and membrane segmentation maps to find touching cells of paired phenotypes.read_components reads component image files.count_within and count_within_batch count the number of from cells having a to cell within a given radius.list_cell_seg_files lists all cell seg data files in a folder.